Journal: eLife

Article Title: Tumor-derived CSF-1 induces the NKG2D ligand RAE-1δ on tumor-infiltrating macrophages

doi: 10.7554/eLife.32919

Figure Lengend Snippet: ( A ) The indicated numbers of B16 or RMA-S cells were seeded in 12-well plates, and CSF-1 levels in the supernatants were measured by ELISA 48 hr later. ( B ) Established B16 or RMA-S tumors were dissociated, and CSF-1 levels in dissociation supernatants were measured by ELISA; intra-tumoral concentrations were calculated using tumor volume measurements (total ng of CSF-1 divided by the tumor volume at time of harvest). ( C ) Peritoneal macrophage RAE-1δ expression 48 hr after culture with concentrated fresh medium, concentrated B16 conditioned medium plus control Ig (1 ug/ml), or concentrated B16 conditioned medium plus anti-CSF-1R (1 ug/ml). ( D ) Peritoneal macrophage RAE-1δ 48 hr after culture with fresh medium, KP conditioned medium plus control Ig, or KP conditioned medium plus anti-CSF-1R (1 ug/ml). Data are representative of >3 independent experiments.

Article Snippet: Cells were washed in complete medium and cultured in 12- or 6-well non-TC-treated cell culture plates (Corning, Corning, NY) for 12–48 hr.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing